The NIH 3D Print Exchange Promotional Reel

This video highlights 3D models which have been produced at the National Institutes of Health. Most of these 3D print files will be freely available for download by the public, through the 3dprint.nih.gov website, along with free access to the segmentation and data processing pipelines, making it easy to directly create custom 3D prints through the website.

Descriptions of the models which appear in the video in order of appearance:

Pink bacteria: cartoon model of Mycobacterium tuberculosis, color indicates mycolic acid coat staining by Ziehl-Neelsen stain (senescent cells stain completely); ATP pumps derived from crystal structure (1PO1), proteasome modeled from crystal structure (2FHG) and cryo-electron microscopy data; cartoon DNA and surface receptors and channels also included; molecular structures not to scale

Green/yellow/white virus: Norwalk virus derived from VIPERdb 1IHM

Orange “potato”: clathrin coated vesicle derived from electron microscopy provided by Bernard Heymann (NCI); orange: clathrin, red: accessory proteins, blue: vesicle

Mixed pastel virus: polyomavirus derived from VIPERdb 1SIE

Orange “knobby” virus: reovirus derived from VIPERdb 1EJ6

Blue/red/yellow “rasta” virus: influenza virus derived from electron microscopy provided by Bernard Heymann (NCI); blue: hemagglutinin, red: neuraminidase; yellow: viral membrane

Purple “knobby” virus: cartoon model of HIV virus; unliganded gp120 trimer derived from EMDB 5019 (purple); RNA capsid (purple cone) derived from data provided by Barbie Ganser-Pornillos (Scripps Research Institute & University of Utah); yellow: viral membrane; red, blue, green: human proteins captured upon viral budding that disguise the virus from the immune system; proteins not to scale; viral halves held together by embedded rare-earth magnets

Purple “pedestal” with pink blobs: HIV “spike” protein (gp120/gp41 trimer) bound by neutralizing antibody b12; both the experimental cryo-electron microscopy (EMDB 5018; purple) and the fitted atomic structure (PDB 3DNL, multiple colors) are depicted; glycosylation (pink) is modeled using the GLYCAM glycoprotein builder

Rainbow dodecahedron: derived from electron microscopy provided by Bernard Heymann (NCI); coloring by radial distance from the center

Large blue & red with big struts: HIV gp120 monomer (red) with glycosylation (cyan) modeled using the GLYCAM glycoprotein builder; neutralizing FAB fragment (green/blue) attached at epitope (yellow) by embedded rare-earth magnets

Hemagglutinin trimer: surface representation of the trimeric structure of influenza hemagglutinin with mutations that influence sialic-acid binding highlighted in purple and blue; sialic acid can be seen bound on the red monomer

Hemagglutinin trimer: ribbon representation of the trimeric structure of influenza hemagglutinin; individual polypeptides are colored separately

Pink & white “club”: Need Description

Microscope: custom lab equipment for studying zebrafish larvae, for the purpose neurogenetic studies provided by the Burgess Lab in NICHD.

The Golgi Apparatus: Cryo-electron tomography from Rachid Sougrat was segmented by Edwin Fung using Amira to create the geometry. This model was originally printed by Darrell Hurt and presented to Jennifer Lippincott-Schwartz before departing for the Golgi Conference in Pavia, Italy, where it was inducted into the Camillo Golgi Museum.

Malaria “teardrop”: cartoon of the merozoite form of Plasmodium falciparum modeled after Bannister’s drawing in http://www.ncbi.nlm.nih.gov/pubmed/11006474

Influenza virus on a pedestal: Terry Yoo’s structure; yellow: hemagglutinin, red: neuraminidase; beige: viral membrane

Visible human: various cutaways of the visible human posed in mid-stride; part of a zoopraxiscope

Visible human head: cutaway of the visible human

Surgical planning model: taken from a patient; white: bone, red: vasculature, black: supporting struts; surgery performed at Walter Reed National Military Medical Center

(View on YouTube)

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